{"id":12025,"date":"2015-01-01T16:53:12","date_gmt":"2015-01-01T16:53:12","guid":{"rendered":"http:\/\/lequia-udg.com\/2015\/01\/01\/monitoring-and-engineering-reactor-microbiomes-of-denitrifying-bioelectrochemical-systems\/"},"modified":"2024-04-19T15:59:38","modified_gmt":"2024-04-19T15:59:38","slug":"monitoring-and-engineering-reactor-microbiomes-of-denitrifying-bioelectrochemical-systems","status":"publish","type":"post","link":"https:\/\/lequia-udg.com\/ca\/2015\/01\/01\/monitoring-and-engineering-reactor-microbiomes-of-denitrifying-bioelectrochemical-systems\/","title":{"rendered":"Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"12025\" class=\"elementor elementor-12025 elementor-6061\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-347f2df6 ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"347f2df6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2841541e ts-bg-color-over-image\" data-id=\"2841541e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6b41d040 ts-align-left elementor-widget elementor-widget-ts_heading\" data-id=\"6b41d040\" data-element_type=\"widget\" data-widget_type=\"ts_heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t<div class=\"ts-heading-subheading ts-reverse-heading-yes animation-style4\"><h4 class=\"ts-custom-heading ts-custom-subtitle\">\r\n\t\t\t\r\n\t\t\t\tAuthors: N. Pous, C. Koch, A. Vil\u00e0-Rovira, M. D. Balaguer, J. Colprim, J. M\u00fchlenberg, S. M\u00fcller, F. Harnisch and S. Puig                                                                                                         \r\n\t\t\t\r\n\t\t\t<\/h4>\r\n\t\t<h2 class=\"ts-custom-heading ts-custom-heading-title\">\r\n\t\t\t\r\n\t\t\t\tMonitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems\r\n\t\t\t\r\n\t\t\t<\/h2>\r\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-18f00f3f ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"18f00f3f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-662c43af ts-bg-color-over-image\" data-id=\"662c43af\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2f4b75e1 elementor-widget-tablet__width-initial elementor-widget elementor-widget-text-editor\" data-id=\"2f4b75e1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p>Denitrifying bioelectrochemical systems (d-BES) are a promising technology for nitrate removal from wastewaters. Microbial community monitoring is required to pave the way to application. In this study, for the first time flow cytometry combined with molecular biology techniques is exploited to monitor and determine the structure\u2013function relationship of the microbiome of a denitrifying biocathode. Stable cathode performance at poised potential (\u22120.32 V <em>vs.<\/em> Ag\/AgCl) was monitored, and different stress-tests were applied (reactor leakage, nitrate concentration, buffer capacity). Stress-tests shifted the reactor microbiome and performance. The monitoring campaign covered a wide range of nitrate consumption rates (from 15 to 157 mg N L<small><sub>NCC<\/sub><\/small><small><sup>\u22121<\/sup><\/small> d<small><sup>\u22121<\/sup><\/small>), current densities (from 0 to 25 mA L<small><sub>NCC<\/sub><\/small><small><sup>\u22121<\/sup><\/small>) and denitrification intermediates (nitrite and nitrous oxide consumption rates varied from 0 to 56 mg N L<small><sub>NCC<\/sub><\/small><small><sup>\u22121<\/sup><\/small> d<small><sup>\u22121<\/sup><\/small>). The reactor microbiome (composed of 21 subcommunities) was characterized and its structure\u2013function relationship was revealed. A key role for <em>Thiobacillus<\/em> sp. in the bioelectrochemical reduction of nitrate was suggested, while a wider number of subcommunities were involved in NO<small><sub>2<\/sub><\/small><small><sup>\u2212<\/sup><\/small> and N<small><sub>2<\/sub><\/small>O reduction. It was demonstrated that different bacteria catalyze each denitrification step in a biocathode. This study contributed significantly to understanding denitrifying biocathodes, paving the way for their knowledge-driven engineering.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6107793c ts-col-stretched-none ts-bg-color-over-image elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6107793c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-585947c1 ts-bg-color-over-image\" data-id=\"585947c1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1c70b942 elementor-widget elementor-widget-accordion\" data-id=\"1c70b942\" data-element_type=\"widget\" data-widget_type=\"accordion.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion\">\n\t\t\t\t\t\t\t<div class=\"elementor-accordion-item\">\n\t\t\t\t\t<div id=\"elementor-tab-title-4771\" class=\"elementor-tab-title\" data-tab=\"1\" role=\"button\" aria-controls=\"elementor-tab-content-4771\" aria-expanded=\"false\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon elementor-accordion-icon-left\" aria-hidden=\"true\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-closed\"><i class=\"fas fa-plus\"><\/i><\/span>\n\t\t\t\t\t\t\t\t<span class=\"elementor-accordion-icon-opened\"><i class=\"fas fa-minus\"><\/i><\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t\t\t<a class=\"elementor-accordion-title\" tabindex=\"0\">Informaci\u00f3 addicional<\/a>\n\t\t\t\t\t<\/div>\n\t\t\t\t\t<div id=\"elementor-tab-content-4771\" class=\"elementor-tab-content elementor-clearfix\" data-tab=\"1\" role=\"region\" aria-labelledby=\"elementor-tab-title-4771\"><table style=\"border: hidden;\">\n<tbody>\n<tr style=\"border-bottom: 1px solid #efefef;\">\n<td align=\"right\" width=\"200\"><strong>Year:<\/strong><\/td>\n<td>2015<\/td>\n<\/tr>\n<tr style=\"border-bottom: 1px solid #efefef;\">\n<td align=\"right\" width=\"200\"><strong>Authors:<\/strong><\/td>\n<td>N. Pous, C. Koch, A. Vil\u00e0-Rovira, M. D. Balaguer, J. Colprim, J. M\u00fchlenberg, S. M\u00fcller, F. Harnisch and S. Puig<\/td>\n<\/tr>\n<tr style=\"border-bottom: 1px solid #efefef;\">\n<td align=\"right\" width=\"200\"><strong>Reference:<\/strong><\/td>\n<td>RSC Advances, 2015, 5, 68326-6833<\/td>\n<\/tr>\n<tr style=\"border-bottom: 1px solid #efefef;\">\n<td align=\"right\" width=\"200\"><strong>Link:<\/strong><\/td>\n<td><a href=\"http:\/\/dx.doi.org\/10.1039\/C5RA12113B\" target=\"_blank\" rel=\"noopener\">http:\/\/dx.doi.org\/10.1039\/C5RA12113B<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2000c3ef elementor-widget elementor-widget-spacer\" data-id=\"2000c3ef\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Authors: N. Pous, C. Koch, A. Vil\u00e0-Rovira, M. D. Balaguer, J. Colprim, J. M\u00fchlenberg, S. M\u00fcller, F. Harnisch and S. Puig Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems Denitrifying bioelectrochemical systems (d-BES) are a promising technology for nitrate removal from wastewaters. Microbial community monitoring is required to pave the way to application. In &hellip; <a href=\"https:\/\/lequia-udg.com\/ca\/2015\/01\/01\/monitoring-and-engineering-reactor-microbiomes-of-denitrifying-bioelectrochemical-systems\/\" class=\"more-link\" title=\"Continue reading <span class=\"screen-reader-text\">Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems<\/span>&#8220;>Continue reading <span class=\"screen-reader-text\">Monitoring and engineering reactor microbiomes of denitrifying bioelectrochemical systems<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[64],"tags":[],"class_list":["post-12025","post","type-post","status-publish","format-standard","hentry","category-articles-ca"],"_links":{"self":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/12025","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/comments?post=12025"}],"version-history":[{"count":1,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/12025\/revisions"}],"predecessor-version":[{"id":12026,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/12025\/revisions\/12026"}],"wp:attachment":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/media?parent=12025"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/categories?post=12025"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/tags?post=12025"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}