{"id":15502,"date":"2026-04-21T10:13:45","date_gmt":"2026-04-21T10:13:45","guid":{"rendered":"https:\/\/lequia-udg.com\/2026\/04\/21\/synergies-revealed-rna-seq-study-of-c-acetobutylicum-and-c-carboxidivorans-co-cultured-in-the-presence-of-conductive-materials\/"},"modified":"2026-04-21T10:17:25","modified_gmt":"2026-04-21T10:17:25","slug":"synergies-revealed-rna-seq-study-of-c-acetobutylicum-and-c-carboxidivorans-co-cultured-in-the-presence-of-conductive-materials","status":"publish","type":"post","link":"https:\/\/lequia-udg.com\/ca\/2026\/04\/21\/synergies-revealed-rna-seq-study-of-c-acetobutylicum-and-c-carboxidivorans-co-cultured-in-the-presence-of-conductive-materials\/","title":{"rendered":"Synergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials"},"content":{"rendered":"\t\t
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\r\n\t\t\t\r\n\t\t\t\tAuthors: \r\n\t\t\t\r\n\t\t\t<\/h4>\r\n\t\t

\r\n\t\t\t\r\n\t\t\t\tSynergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials\r\n\t\t\t\r\n\t\t\t<\/h2>\r\n\t\t<\/div>\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t
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Co-cultures can improve substrate utilization and product yields, yet the dynamics between species remain highly variable and poorly understood. In this study, we investigated the metabolic and transcriptional interplay between\u00a0Clostridium acetobutylicum<\/em>\u00a0and\u00a0Clostridium carboxidivorans<\/em>\u00a0when co-cultured in the presence of activated carbon or magnetite, with the aim of evaluating these materials as metabolism enhancers. Fermentation profiling showed that magnetite accelerated glucose consumption and favored acid over alcohol production, with butyrate and acetate reaching yields of 0.65 and 0.62 mol\/mol glucose, respectively. Alcohols (ethanol and butanol) accumulated in late fermentation and occurred concomitantly to a metabolic shift from acidogenesis to solventogenesis, potentially driven by interspecies dynamics as RNA-seq data suggested. RNA-seq analysis detected 7,369 genes and revealed\u00a0C. carboxidivorans<\/em>\u00a0dominated in early fermentation, and\u00a0C. acetobutylicum<\/em>\u00a0was activated later (in view of the number of reads detected for each species). Magnetite-treated samples displayed the most transcriptional variation, and species-specific patterns emerged. Changes in electron-active genes (e.g.,\u00a0hydA<\/em>\u00a0and\u00a0rnf<\/em>\u00a0genes) suggest enhanced redox communication, highlighting the capacity of conductive materials to influence metabolic flow and regulatory pathways in microbial consortia, opening possibilities for improved bioproduction and carbon utilization.<\/p>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t

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Year:<\/strong><\/td>\n2026<\/td>\n<\/tr>\n
Authors:<\/strong><\/td>\nL. Feliu-Paradeda, Q. Amor\u00f3s-Espu\u00f1a, E. Perona-Vico, E. Casals, A. Esteve-Codina, S. Puig, L. Ba\u00f1eras<\/td>\n<\/tr>\n
Reference:<\/strong><\/td>\nMicrobiology Spectrum, Volume 14, Issue 4, 2026<\/td>\n<\/tr>\n
Link:<\/strong><\/td>\nhttps:\/\/doi.org\/10.1128\/spectrum.03262-25<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t
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Authors: Synergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials Co-cultures can improve substrate utilization and product yields, yet the dynamics between species remain highly variable and poorly understood. In this study, we investigated the metabolic and transcriptional interplay between\u00a0Clostridium acetobutylicum\u00a0and\u00a0Clostridium carboxidivorans\u00a0when co-cultured in the presence of … Synergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials<\/span>“>Continue reading Synergies revealed: RNA-seq study of C. acetobutylicum and C. carboxidivorans co-cultured in the presence of conductive materials<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"elementor_header_footer","format":"standard","meta":{"footnotes":""},"categories":[64],"tags":[],"class_list":["post-15502","post","type-post","status-publish","format-standard","hentry","category-articles-ca"],"_links":{"self":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/15502","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/comments?post=15502"}],"version-history":[{"count":1,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/15502\/revisions"}],"predecessor-version":[{"id":15503,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/posts\/15502\/revisions\/15503"}],"wp:attachment":[{"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/media?parent=15502"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/categories?post=15502"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/lequia-udg.com\/ca\/wp-json\/wp\/v2\/tags?post=15502"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}